The aim of this study was to study the effect of miR-375 on non-small cell lung carcinoma  (NSCLC) invasion, migration, and proliferation through the CIP2A pathway.

We constructed a stable over-expressing cell line with lentivirus as the experimental group  (Lv-miR-375) and transfected the empty vector as the negative control group (Lv-NC). The expression level  of miR-375 was detected using real-time fluorescence quantitative PCR (qRT-PCR).Western blots were  used to detect the expression levels of cancerous inhibitor of PP2A (CIP2A), MYC, protein kinase B (AKT)  and p-AKT in Lv-NC- and Lv-miR-375-transfected cells. Transwell assays were conducted to detect the  cell invasion and metastasis ability, and the cell counting kit-8 (CCK8) was used to detect cell proliferation.

qRT-PCR showed that miR-375 was overexpressed in NSCLC. Compared to the Lv-NCtransfected cells, the western blot results showed that CIP2A, MYC and p-AKT were highly expressed in  Lv-miR-375-transfected cells. Transwell assays showed that the invasion and migration ability of Lv-miR- 375-transfected A549 cells was significantly higher than that of Lv-NC-transfected cells. CCK8 experiments  showed that compared to Lv-NC-transfected cells, the cell proliferation ability of the Lv-miR-375-transfected  cells increased.

MiR-375 could promote the invasion, migration, and proliferation of NSCLC A549 cells via  the CIP2A pathway. MiR-375 is expected to become a new target for the treatment of NSCLC, and may  become an important biomarker for the diagnosis, prognosis, and treatment of the disease.